🌿 Soil pH determination

Environmental PN-ISO 10390

In short

Potentiometric determination of soil pH in suspension with water (pH_H₂O) or KCl solution (pH_KCl). The test is performed according to PN-ISO 10390:2022; Measurement range: 2–12 pH. The procedure comprises 7 steps (about 2 h 25 min in total); it is used for: Agricultural soil monitoring (OSChR, IUNG), Fertilization recommendations (liming), Soil quality assessment according to Polish regulation.

At a glance

  • Standard: PN-ISO 10390:2022
  • Category: Environmental
  • Procedure steps: 7
  • Total time of stages: 2 h 25 min
  • Measurement range: 2–12 pH
  • Soil:liquid ratio: 1:5 (v/v)
  • KCl solution: 1 mol/L

Overview

Soil pH is one of the most important agrochemical parameters, affecting nutrient availability for plants, soil microorganism activity, and heavy metal mobility. Two values are determined: pH_H₂O (actual pH, in water suspension) and pH_KCl (exchangeable acidity, in 1 mol/L KCl suspension).

pH_KCl is lower than pH_H₂O by 0.5–1.5 units and better reflects soil buffering properties, as K⁺ ions displace H⁺ and Al³⁺ from the sorption complex. Agronomic soil pH classes: very acidic <4.5; acidic 4.5–5.5; slightly acidic 5.6–6.5; neutral 6.6–7.2; alkaline >7.2.

The method is simple, fast, and does not require expensive equipment, which is why it is performed en masse by Regional Chemical-Agricultural Stations (OSChR) in agricultural soil monitoring.

Method principle

The soil sample is mixed with distilled water or 1 mol/L KCl solution in a volume ratio of 1:5 (soil:liquid). The suspension is vigorously mixed for 5 minutes, left for 2 hours to equilibrate, and then pH is measured potentiometrically using a pH meter with combined glass electrode. Two-point calibration with pH 4.00 and 7.00 buffers.

Applications

Key parameters

ParameterValue
Measurement range2–12 pH
Soil:liquid ratio1:5 (v/v)
KCl solution1 mol/L
Equilibration time2 h (±15 min)
Accuracy±0.1 pH

Standard

Standard number
PN-ISO 10390:2022
Title (PL)
Jakość gleby — Oznaczanie pH
Title (EN)
Soil quality — Determination of pH

Step-by-step procedure

  1. Sample preparation

    Dry soil at room temperature. Sieve through 2 mm sieve. Measure 5 mL soil with graduated cylinder.

    ⏱ Time: 10 min

  2. Suspension preparation

    To 5 mL soil add 25 mL distilled water (pH_H₂O) or 25 mL 1 mol/L KCl (pH_KCl).

    ⏱ Time: 2 min

  3. Mixing

    Shake vigorously for 5 minutes on shaker (150–200 rpm) or manually.

    ⏱ Time: 5 min

  4. Equilibration

    Leave suspension for 2 h (±15 min) at room temperature. Do not mix during this time.

    ⏱ Time: 2 h

  5. pH meter calibration

    Calibrate pH meter with 4.00 and 7.00 buffers. Check slope (95–105% of theoretical).

    ⏱ Time: 5 min

  6. pH measurement

    Immerse electrode in suspension (2 cm below surface). Wait for stabilization (±0.02 pH for 30 s). Record result.

    ⏱ Time: 2 min

  7. Rinsing and repetition

    Rinse electrode with distilled water between samples. Perform duplicate every 10 samples.

    ⏱ Time: 1 min

Required equipment and apparatus

EquipmentExampleIndicative price
Laboratory pH meterMettler Toledo SevenCompact S220, WTW inoLab pH 73103,000–15,000 PLN
Combined pH electrodeMettler Toledo InLab Expert Pro, WTW SenTix 940500–2,000 PLN
Laboratory shakerIKA KS 130 basic, Heidolph Unimax 10103,000–8,000 PLN
PP beakers 100 mLDisposable polypropylene beakers50–100 PLN/100 pcs.

Reagents, media and consumables

ReagentCASDetails
Potassium chloride KCl7447-40-71 mol/L solution (74.55 g/L), analytical grade
pH buffers 4.00 and 7.00Certified CRM for pH meter calibration
Distilled waterConductivity <0.2 mS/m, pH 5.6–6.5

Health and safety (OHS)

Frequently asked questions

Which standard describes this test?

The test is performed according to PN-ISO 10390:2022 — “Soil quality — Determination of pH”.

How does this method work?

The soil sample is mixed with distilled water or 1 mol/L KCl solution in a volume ratio of 1:5 (soil:liquid). The suspension is vigorously mixed for 5 minutes, left for 2 hours to equilibrate, and then pH is measured potentiometrically using a pH meter with combined glass electrode.

What is the measuring range and accuracy?

Measurement range: 2–12 pH; Soil:liquid ratio: 1:5 (v/v); KCl solution: 1 mol/L; Equilibration time: 2 h (±15 min).

How long does the test take?

The times given for the individual stages add up to approximately 2 h 25 min. The procedure comprises 7 steps.

What equipment is required?

Laboratory pH meter, Combined pH electrode, Laboratory shaker, PP beakers 100 mL. Indicative cost of the core instrument (Laboratory pH meter): 3,000–15,000 PLN.

Where is this test used?

Agricultural soil monitoring (OSChR, IUNG); Fertilization recommendations (liming); Soil quality assessment according to Polish regulation; Assessment of fertilization and cultivation impact on soil pH; Assessment of heavy metal mobility in soil; Environmental studies — acid rain impact.

What safety precautions apply?

KCl — harmless, standard protective measures; Glass electrode — fragile, risk of cuts; Gloves and safety goggles — standard equipment; Soil may contain contaminants — use nitrile gloves.

Which laboratory can perform this test?

The test is performed by laboratories accredited to ISO/IEC 17025. On LabCoda you can find them by the standard number PN-ISO 10390.

🔍 Find a laboratory performing this test